4In 2007, Barrangou et al. demonstrated for the first time the function of CRISPR/Cas9 as a defensive mechanism of bacteria against bacteriophage attacks by a technique called “plaquing” which builds upon the technique of “plating” bacteria on agar. Plaques of viruses on agar are areas without growth of bacteria due to viral attacks.
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It was partly inspired by To Hunt a Killer, a book written by crime correspondent Robert Murphy about Det Supt Julie Mackay's 2009 cold case investigation, 32 years after the murder of Melanie Road as she walked home from a nightclub in Bath in 1984.
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